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. 2021 Sep 1;12:5230. doi: 10.1038/s41467-021-25444-3

Fig. 4. Electrophysiological recordings showed a reduction in evoked APs in M/T as well as altered synaptic properties.

Fig. 4

(a–d) Whole-cell patch clamp electrophysiology of M/T cells revealed a reduction in firing number from stepped current injections with Sox9 KO in astrocytes vs control astrocytes. n = 13 cells (control) and n = 12 cells (Sox9 KO) from N = 3 mice, respectively. **p = 0.0023 and 0.0014 for 100 and 120 pA current injections, respectively; ****p < 0.0001; Two-way ANOVA with Sidak’s multiple comparison correction. Data are presented as box plots displaying interquartile range and median with Tukey whiskers. e Example traces of mEPSC recordings in M/T cells with control astrocytes (top) and Sox9 KO astrocytes (bottom). f M/T cells with Sox9 KO astrocytes show a significant decrease in mEPSC frequency compared to control astrocytes. n = 8 cells (control) and n = 7 cells (Sox9 KO) from N = 3 mice, respectively. *p = 0.0156. Two-tailed Student’s t-test. Data are presented as mean values ± SEM. g M/T cells show no changes in mEPSC amplitude with Sox9 KO in astrocytes. n = 8 cells (control) and n = 7 cells (Sox9 KO) from N = 3 mice, respectively. Two-tailed Student’s t-test. h Example traces of mIPSC recordings in M/T cells with control astrocytes (top) and Sox9 KO astrocytes (bottom). Data are presented as mean values ± SEM. i M/T cells with Sox9 KO astrocytes show a significant decrease in mIPSC frequency compared to control astrocytes. n = 7 cells (control) and n = 7 cells (Sox9 KO) from N = 3 mice, respectively. *p = 0.0392. Two-tailed Student’s t-test. Data are presented as mean values ± SEM. j M/T cells show no changes in mIPSC amplitude with Sox9 KO in astrocytes. n = 7 cells (control) and n = 7 cells (Sox9 KO) from N = 3 mice, respectively. Two-tailed Student’s t-test. Control is C57BL/6 J injected with AAV-GFAP-iCre-P2a-TurboRFP. Data are presented as mean values ± SEM.