Table 3.
qRT-PCR results at week 13.
| Gene/house-keeping gene | Groups | |||
|---|---|---|---|---|
| Sham | CKD | CKD + losartan | CKD + mirabegron | |
| Myh6/Ppia | 1.41 ± 0.07 | 1.23 ± 0.11 | 1.21 ± 0.07 | 0.76 ± 0.09*# |
| Myh7/Ppia | 1.29 ± 0.1 | 1.27 ± 0.18 | 0.71 ± 0.12*# | 1.01 ± 0.14 |
| Myh6/Myh7 | 0.94 ± 0.09 | 1.26 ± 0.15 | 0.61 ± 0.13# | 1.32 ± 0.31 |
| Col1a1/Ppia | 0.2 ± 0.05 | 0.32 ± 0.08 | 0.25 ± 0.04 | 0.19 ± 0.06 |
| Ctgf/Ppia | 0.93 ± 0.09 | 1.32 ± 0.15* | 0.82 ± 0.08# | 1.91 ± 0.24*# |
| Nppa/Ppia | 0.42 ± 0.09 | 0.63 ± 0.15 | 0.24 ± 0.02# | 0.56 ± 0.05 |
| Nppb/Ppia | 1.05 ± 0.15 | 1.23 ± 0.11 | 0.64 ± 0.09# | 1.19 ± 0.16 |
| Nox4/Ppia | 1.75 ± 0.18 | 2.38 ± 0.20 | 0.57 ± 0.09*# | 1.25 ± 0.13*# |
| Nos2/Ppia | 0.95 ± 0.19 | 2.80 ± 0.55* | 1.26 ± 0.21# | 1.17 ± 0.37# |
| IL1/Ppia | 0.77 ± 0.05 | 1.18 ± 0.11* | 0.78 ± 0.06# | 0.94 ± 0.16 |
| IL6/Ppia | 0.81 ± 0.18 | 2.94 ± 0.6* | 0.37 ± 0.08# | 0.52 ± 0.12# |
| Tnf-α/Ppia | 0.77 ± 0.05 | 1.2 ± 0.1* | 0.83 ± 0.09# | 0.99 ± 0.12 |
| Agtr1a/Ppia | 1.55 ± 0.06 | 1.13 ± 0.1* | 1.26 ± 0.11 | 0.86 ± 0.08*# |
| Agt/Ppia | 1.32 ± 0.09 | 1.24 ± 0.09 | 1.08 ± 0.12 | 1.12 ± 0.14 |
| Adrb3/Ppia | 0.65 ± 0.10 | 0.99 ± 0.07 | 0.9 ± 0.1 | 1.08 ± 0.21 |
Values are presented as mean ± S.E.M., *p < 0.05 vs. sham-operated group, #p < 0.05 vs. CKD group (n = 6–10, One-Way ANOVA, Holm-Sidak post hoc test).
Sham sham-operated group, CKD chronic kidney disease group, Adrb3 β-3 adrenergic receptor, Agt angiotensinogen, Agtr1a angiotensin-II receptor type 1a, Col1a1 collagen type 1 alpha 1 chain, Ctgf connective tissue growth factor, IL1 interleukin-1, IL6 interleukin-6, Myh6 α-myosin heavy chain, Myh7 β-myosin heavy chain, Nos2 inducible nitric oxide synthase, Nox4 NADPH-oxidase type 4, Nppa A-type natriuretic peptide, Nppb B-type natriuretic peptide, Ppia Peptidyl prolyl isomerase A, Tnf-α tumor necrosis factor alpha. Ppia was used as a housekeeping gene for normalization.