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. 2021 Sep 1;11:17496. doi: 10.1038/s41598-021-96899-z

Table 2.

Alpha diversity of microbiome evenness, richness and varieties of species in the sediments.

Bacteria Eukarya
Biofilm water (AMD-EB) Enrichment culture (AMD-EC) Biofilm water (AMD-EB)
Actual
Valid reads 26,160 26,373 40,403
OTUs 2036 95 1002
Estimated richness
ACE 2293.26 133.37 1033.01
 HCI 2346.43 169.09 1047.66
 LCI 2244.59 109.57 1019.11
Chao1 2174.97 126.23 1016.11
 HCI 2217.70 173.29 1029.93
 LCI 2142.28 107.46 1009.13
JackKnife 2438 127.45 1078
 HCI 2438 145.15 1078
 LCI 2438 109.75 1078
Estimated diversity
NPShannon 5.97 1.58 3.54
Shannon 5.86 1.57 3.51
 HCI 5.88 1.59 3.54
 LCI 5.84 1.55 3.48
Simpson 0.01 0.41 0.13
 HCI 0.01 0.42 0.14
 LCI 0.0097 0.41 0.13
Phylogenetic diversity 2779 182 1186
Good’s Lib. coverage (%) 98.45 99.89 99.81

Clustering of OTUs found was achieved with UCLUST and the open reference method as all taxa were selected for analysis.

HCI High Confidence Interval (95%), LCI Low Confidence Interval (95%), OTUs Operational taxonomic units determined at 97%.