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. 2021 Sep 1;11:17527. doi: 10.1038/s41598-021-96838-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of tumor burden in a pancreatic cancer mouse. Three-dimensional volume rendering is shown in (a). Mouse body, liver, and metastases are shown in white, red, and green, respectively. The number of metastases in liver and the rest of the body are shown in (b). The two plots in (c) represent the histogram of radius of metastases in the liver, and the rest of the body with the same colors as the plot in (b). All metastases in the liver are smaller than 1.5 mm, and there is a large metastasis with radius 4.0 mm in the pancreas. Representative metastases in the liver, pancreas, and lymph nodes are shown in (d). For lymph nodes, the left one is the brachial and the right one is the inguinal. Scale bar is 100 µm.