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. 2021 Sep 1;11:17527. doi: 10.1038/s41598-021-96838-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of tumor burden in four breast cancer mice. Three-dimensional volume rendering of the four cancer mice are shown on the left. Mouse body, brain, liver, lung, and metastases are shown in white, yellow, blue, red, and green, respectively. In the middle, the number of metastases in lung, liver, brain, and the rest of the body are shown. The right four subplots represent the histogram of radius of metastases in the lung, liver, brain, and the rest of the body with the same colors as the plot in the middle. There are no metastases bigger than 2.5 mm in cancer mouse 1–3, and there are 2 metastases in the range of 2.5–3 mm in mouse 4.