Fig. 3. Correlation between CTL Ca2+ signaling and perforin pore formation in different target cells.

a Percentage of CTL contacts associated with Ca²⁺ events in OVA-expressing target cells (OVA) compared to OVA-negative parental (par) cells. Data show the means ± SD from three independent experiments. b Image sequence of Fura2-labeled OT1 CTL during contact with GCaMP6s-expressing B16F10/OVA cell. Arrowheads: green, Fura2-positive event; yellow, GCaMP6s-positive event. Scale bar, 10 μm. c Percentage of contacts triggering Ca²⁺ events in OT1 CTL or B16F10/OVA target cells, respectively. Quantification (means and SD) was performed by manual analysis from 78 contacts pooled from N = 4 independent experiments. Source data are provided as a Source Data file.