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. 2021 Sep 1;5:26. doi: 10.1038/s41538-021-00109-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Upper panel: a Control, no peptide; b HSEP3 treated at 0.5X MIC concentration, and c HSEP3 treated at 1X MIC concentration. All three samples treated with Syto-9 (in green, indicating live cells) and propidium iodide (in red, indicating dead cells). d Permeabilization of the cytoplasmic membrane of B. cereus as a function of HSEP3 peptide concentrations (0–100 μg/mL), calculated as percent of propidium iodide fluorescence. e Permeabilization of the cytoplasmic membrane of B. cereus by HSEP2, HSEP2-K8G, and HSEP2-∆K8 (2X MIC), indicated by percent of propidium iodide fluorescence. Data represent mean ± s.e.m. of three biological replicates. Scale bar denotes 25.40 μm.