Table 1.

Peptides used in this study. Amino acid sequences, molecular weights, net charges, length, MICs (against M. luteus) and Ki values (against bovine trypsin) of the tested peptides.

	SN	Name	Sequence	Charge	Length	MIC (µg/mL) M. luteus	Ki (M)	MW in Daltons
	1	SFTI-cyclic	GRCTKSIPPICFPD	+2	14	–	5.5 ± 0.18 × 10−8	1513.6
Box A	2	HVBBI	SVIGCWTKSIPPRPCFVK	+3	18	150	3.7 ± 0.11 × 10−7	2016.6
	3	SFTI-loop	CTKSIPPICF	+1	10	–	0.5 ± 0.19 × 10−6	1108.3
	4	HVBBI-loop	CWTKSIPPRPCF	+2	12	>150	1.4 ± 0.23 × 10−6	1434.6
	5*	HSEP1	SVIGCTKSIPPICFVK	+2	16	75	2.3 ± 0.14 × 10−7	1690.2
Box B	6*	HSEP2	SVIFGCTKSIPPICFVGFK	+2	19	6.25	5.8 ± 0.32 × 10−7	2041.5
	7*	HSEP3	RSVIFGCTKSIPPICFVGFK	+3	20	1.25	2 ± 0.47 × 10−7	2197.6
	8*	FITC-HSEP2	FITC-SVIFGCTKSIPPICFVGFK	+2	19	6.25	–	2543.8
	9*	HSEP2-∆K8	SVIFGCTISIPPICFVGFK	+1	18	>150	NI	1912.6
Box C	10*	HSEP2-K8G	SVIFGCTGSIPPICFVGFK	+1	19	37.5	NI	1969.7
	11*	HSEP2-∆K19	SVIFGCTKSIPPICFVGFI	+1	18	25	4.4 ± 0.12 × 10−7	1913.3
	12*	HSEP2-ΔHR	AICTKSIPPICGIK	+2	12	>150	1 ± 0.09 × 10−6	1215.5
	13*	HSEP2-ΔHR- ΔK8	AICTISIPPICGIK	+1	11	>150	NI	1087.3
	14*	HSEP3-∆TL,CL+	RSVIFGCYRRFCFVGFK	+4	17	3.125	NI	2083.40
	15*	HSEP3a	RSFIFGCTKSIPPICFVGFK	+3	20	12.5	5.5 ± 0.13 × 10−8	2245.50
Box D	16*	HSEP3b	RSVIFGCTKSIPPICFVGTR	+3	20	6.25	0.5 ± 0.38 × 10−6	2179.35
	17*	HSEP3c	RSVIFGCTKSKIPPICFVGFK	+4	21	3.125	1.4 ± 0.25 × 10−6	2398.35
	18*	HSEP3d	RSWIFCTRYIPPICFVGWR	+3	19	3.125	3.7 ± 0.16 × 10−7	2325.40

Underlined residues are the ones that are mutated with respect to the mother peptide.

*Peptides engineered for this study. NI, No Inhibition.

^IImplies residues deleted from the mother peptide (e.g., those with ΔK).