Fig. 3. Physicochemical characteristics of CD47 TMD core.
a–b Two views of the TMD of CD47BRIL-B6H12 crystal structure (gray cartoon, helices numbered I-V, ECD and Fab atoms omitted) showing residues in the core of the TMD helix bundle and in the ECLR. The surface of TMD hydrophobic residues pointing towards the core of the receptor are shown in light orange. The side chains of residues in the 114RVVSWF119 linker are shown as green sticks. Hydrogen bonds are represented by gray dotted lines. The asterisk indicates the position of the inter-domain disulfide bond between C15 and C245. Black ellipses delineate the IC region containing the cluster of hydrophobic residues between helices I, II, and III (HC2, shown in (a)), and residues involved in the IC hydrogen bond network (shown as purple transparent surfaces and sticks in (b)). ECL1 and ECL2 are colored orange and gray respectively and are labeled. Disordered ICL2 and C-terminal residues are represented by red and black dotted lines respectively. c–e Top views from the CD47 EC side showing the packing of hydrophobic residues in the receptor core and the different layers (delineated by the rectangles in (b)), from the outer membrane leaflet to the center of lipid bilayer.