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. 2021 Sep 1;12:5206. doi: 10.1038/s41467-021-25541-3

Fig. 3. Characteristics of CRISPR-Prime Editing system for DNA engineering in E. coli.

Fig. 3

The editing efficiency was defined as ratio of white clones (GFP-negative)/ total clones on a screening plate. a. Eight different concentrations of ATc, ranging from 0 to 1000 ng/mL (0, 10, 20, 50, 100, 200, 500, and 1000) were used to evaluate the induction of CRISPR-Prime Editing system on four DNA engineering events of 3 bp insertion, 1 bp deletion, 1 bp substitution, and the combinatorial editing. b The evaluation of PBS length. c The evaluation of RTT scaffold length. d The capacity of DNA fragment insertion with different sizes. e The capacity of DNA fragment deletion with different sizes. Mean ± s.d. of three biological replicates are shown. 200 ng/mL of ATc was used for b–e.