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. 2021 Sep 1;7:33. doi: 10.1038/s41526-021-00163-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a H3K27me3 levels were analyzed in the intergenic region of the T40D4.12-4.10 locus on Chromosome V. The red-colored gene is a gene containing the DUF-19 domain. b The graphs show the recovery rates for region #01 and #02, as a relative ratio of DNA after ChIP with the anti-H3K27me3 monoclonal antibody compared to the input DNA in adults wild-type and hda-4 mutant, respectively. Due to the sample limitations, L1 larvae study was limited to analysis of the H3K27me3 status at the region #01. These samples (about 300 worms each) grew as third generation under artificial 1G or microgravity conditions. Biological triplications in each sample condition (n = 3) were analyzed by ChIP-qPCR using one-way ANOVA followed by Tukey post hoc test. Error bars indicate S.D.