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. 2021 Apr 6;31(9):951–964. doi: 10.1038/s41422-021-00499-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative TEM images showing vesicles beneath the plasma membrane of atrial cardiomyocytes (yellow arrows). Scale bar, 200 nm. b The immunofluorescence confocal images showing glutamate (red) colocalized with the secretory vesicles marker, CAST (green) in atrial cardiomyocytes. Scale bar, 10 μm. c 3D reconstruction from the z-stack confocal image showing colocalization of glutamate and CAST in the atrial cardiomyocyte. Left, 3D view of the whole cell labeled with glutamate antibody (red) and CAST antibody (green). Right, 2D section view of the x-z coordinates showing the colocalization (white arrows) of glutamate (red) and CAST (green) in the atrial cardiomyocyte.