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. 2021 Jul 27;17(3):503–514. doi: 10.1007/s11302-021-09806-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Distribution of adenosine A₁ receptors (A₁R) in dorsal root ganglia (DRG). a Fluorescent in situ hybridization detected A₁R mRNA in mouse DRGs. b No hybridization signal was detected using a scramble control probe. c–e Fluorescent in situ hybridization of A₁R mRNA combined with immunostaining of calcitonin gene-related peptide (CGRP; c), binding of isolectin B4 (IB4; d), or immunostaining of neurofilament-200 (NF200; e) revealed that A₁R mRNA is expressed in populations of peptidergic and non-peptidergic C fibers and myelinated DRG neurons, respectively. f Quantitative summary of DRG neuron populations expressing A₁R (2061 cells counted; n = 3 animals). Scale bars: 100 µm (a), 25 µm (c)