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. 2021 Jul 20;17(3):481–492. doi: 10.1007/s11302-021-09798-3

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© The Author(s) 2021

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Fig. 1 — Purinergic receptor expression in bone marrow-derived macrophages (BMDMs) from C57Bl6/J mice. Flow cytometric analysis of a directly isolated bone marrow and b differentiated 7 days with 30 ng/ml M-CSF. Lineage describes a combination of antibodies recognizing CD3, CD19, Ly6G, and NK1.1, respectively. Macrophages are characterized as CD45⁺, CD11b⁺, and F4/80⁺ and lineage negative population. c Real-time PCR purinergic receptor and ectonucleotidase expression profile of unstimulated BMDMs described as 2^-dCt values. Red dotted lines visualize the threshold of very low expressional levels. n.d., not detectable