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. 2021 Sep 1;12:5204. doi: 10.1038/s41467-021-25546-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Experimental scheme for adenoviral expression of Sec61b-V5-TurboID and biotin labeling in mouse liver tissue. b Immunofluorescence imaging of TurboID (Anti-V5) and biotinylated proteins (Streptavidin-Alexa) in liver tissue sections from GFP or Sec61b-V5-TurboID adenovirus (AdV) transduced mice. Scale bars, 50 μm. These experiments were repeated as biological triplicates with similar results. c, d Streptavidin-HRP detection of biotinylated proteins (c) and Ponceau S staining of proteins (d) in blood plasma from GFP or Sec61b-V5-TurboID adenovirus (AdV) transduced mice. These experiments were repeated as biological triplicates with similar results. e Biotinylated secretory protein profiles generated by Sec61b-V5-TurboID adenovirus in supernatants of hepatocyte cell lines, HepG2 and AML12, and plasma of liver iSLET mice. These experiments were repeated as biological triplicates with similar results. f Relative abundance of biotinylated secretory proteins detected in the plasma of liver iSLET mice in terms of biotinylated spectral counts. ALB, Serum albumin; PZP, Pregnancy zone protein; TF, Serotransferrin; SERPINA3K, Serine protease inhibitor A3k; MUG1, Murinoglobulin-1; FGA, Fibrinogen alpha chain; APOA1, Apolipoprotein A-I; FGG, Fibrinogen gamma chain; HPX, Hemopexin. g Specificity analysis for biotinylated proteins with SignalP 5.0, Human Protein Atlas (HPA), and literature.