Skip to main content
. 2021 Aug 31;135(17):2049–2066. doi: 10.1042/CS20210575

Figure 2. Placenta-specific Slc38a2 knockdown reduces TPM SNAT2 abundance and placental System A amino acid transport.

Figure 2

(A) SNAT2 protein abundance in isolated TPMs from pooled SCR and Slc38a2KD placentas, determined by Western blot. Representative blot includes human placental microvillous membrane (MVM) sample, as positive control. (B–E) Placental uptake and transplacental transport of 14C-MeAIB in SCR (n=13 litters, representing 46 conceptuses) and Slc38a2KD conceptuses (n=13 litters, representing 58 conceptuses), expressed as absolute values (B,C) and per gram of placenta (D,E). Litter mean values for SCR and Slc38a2KD conceptuses compared by paired t test. P-values for significant differences (P<0.05) given in figure. Mean + SEM. (F) Correlation of fetal weight with placental 14C-MeAIB uptake for SCR (n=46) and Slc38a2KD (n=58) conceptuses. Points represent individual conceptuses. Pearson correlation coefficient (R) and P-value given in figure. Linear regression line with 95% confidence intervals shown.