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. 2021 Aug 31;71:103547. doi: 10.1016/j.ebiom.2021.103547

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig 1 — Computerized image analysis of CD8⁺ T-lymphocytes in digital tissue slides. (a) Overview of one representative tissue core with CD8 (Leica Biosystems Cat# NCL-CD8-4B11, RRID:AB_442068) immunohistochemical staining. (b) Positive (brown) and negative (counterstained blue) nuclei were shown on magnified image of one subarea. (c) Computerized image analysis was performed by using the software (Immunopath), where positive nuclei were marked with green dots, whereas the negative nuclei were marker with red dots. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article).