Fig. 3.
Apoptotic induction of AK-I-191. (A) Cell cycle analysis of AK-I-191-treated cells. G1 arrest was observed in AK-I-191 concentration- and time-dependent manner. ***p<0.001, **p<0.01 and *p<0.05 are significantly different from control without compound. (B) Western blot analysis of G1 checkpoint markers, cyclin D1 and p27kip1 in T47D cells after treatment of AK-I-191. (C) Apoptosis assessment using Annexin V-PI double staining. T47D cells were treated with varying concentrations of AK-I-191 for 36 h. Apoptotic cells were detected by flow cytometry and plotted as Annexin V-positive cells on the right side. (D) Assessment of Apoptotic induction with changes in protein levels. Protein levels of pro- and anti-apoptotic proteins, bcl-2, bax, and cleaved PARP, were analyzed using western blotting.