Skip to main content
. 2021 Aug;10(8):2500–2510. doi: 10.21037/gs-21-481

Figure 5.

Figure 5

CircRAD18 regulates papillary thyroid cancer metabolism reprograming through the circRAD18-miR-516b-PDK1 axis. (A) Predicted interacting sequences of miR-516b within the 3’-UTR of PDK1 mRNA. (B) PDK1 expression in papillary thyroid cancer cell lines. (C) Luciferase reporter assay of TPC-1 and KAT-5 cells co-transfected with miR-516b mimics and the wild type/mutant 3’-UTR of PDK1 luciferase reporter. (D) Expression level of PDK1 was reduced after transfection with miR-516b mimics. (E) Gathering of circRAD18, PDK1, and miR-516b on AGO2. (F) Abundance of AGO2 to circRAD18 was reduced, while PDK1 was enhanced after knockdown of circRAD18. **, P<0.01.