Figure 6.

Cofilin-1 is involved in the muscle force generation in vivo

(A) Schematic representation of the experimental procedure followed for transduction with AAV vectors expressing cofilin-1 constructs of soleus muscles in young WT mice.

(B) Representative immunoblot of cofilin-1 protein levels in soleus from WT mice non-injected or injected with either PBS or AAV vector expressing cofilin-1 constructs. GAPDH is shown as loading control.

(C) Representative immunoblot showing the effect of AAV expressing cofilin-1 construct on G-actin and F-actin expression in the soleus from WT mice non-injected or injected with either PBS or AAV vector expressing cofilin-1 constructs.

(D) Tetanic force of soleus from WT (n = 17), Lmna^{p.H222P/H222P} (H222P) (n = 9) and WT mice injected with either PBS (n = 4) or AAV vectors expressing cofilin-1 (n = 7), cofilin-1(T25A) (n = 3) or cofilin-1(T25D) (n = 3). ^∗p ≤ 0.01 between WT and Lmna^{p.H222P/H222P} (H222P), ^∗∗p ≤ 0.001 between WT and WT AAV vectors expressing cofilin-1, ^∗∗∗p ≤ 0.0001 between WT and WT AAV vectors expressing cofilin-1(T25D). Data are represented as mean ± SD.

(E) Sirius Red staining of cross sections of soleus muscles from WT mice non-injected or injected with either PBS or AAV vector expressing cofilin-1 constructs. Section of soleus muscle from Lmna^{p.H222P/H222P} (H222P) is shown as control. Scale bar, 50 μm.

(F) Expression of fibrosis-related genes (Col1a2, Col3a1, and Ctgf) in the soleus from WT mice non-injected or injected with either PBS or AAV vector expressing cofilin-1 constructs. Quantification of soleus muscle from Lmna^{p.H222P/H222P} (H222P) is shown as control. ^∗p ≤ 0.01 between WT and Lmna^{p.H222P/H222P} (H222P). Data are represented as mean ± SD.