Figure 2.

Validation of the conditional-inducible Zeb1 knockout model

(A) Breeding strategy to generate inducible control and Zeb1 knockout mice with endogenous tdTOM reporter expression.

(B) Tamoxifen (TAM) was administered daily for 5 consecutive days. Tissue was harvested at the indicated time points post-induction (black arrows).

(C) Induction of tdTOM in GFAP⁺ RGL cells was comparable in both models.

(D) Representative image depicting overlap of tdTOM in GFAP⁺ RGL cells within the SGZ.

(E) Representative images showing RGL cells are ZEB1⁺ in control mice (arrow), but ZEB1⁻ following TAM administration in Zeb1^−/− mice (arrow).

(F) Quantification of ZEB1 expression in GFAP⁺tdTOM⁺ RGL cells in the SGZ of control and Zeb1^−/− mice 1 day post-induction.

(G) Comparison of ZEB1-expressing RGL cells at 4 weeks post-induction in control, Zeb1^+/−, and Zeb1^−/− mice.

Dots represent individual mice (minimum of two sections analyzed per animal); numerical data are shown as mean ± SEM. Scale bars: 100 μm (D); 20 μm (F); 10 μm (insets).