Figure 4.

Effects of Zeb1 loss in newborn neurons

(A) Representative images of DCX⁺tdTOM⁺ neuroblasts (arrows) at 2 weeks post-induction.

(B) Quantification of DCX⁺tdTOM⁺ neuroblasts in the DG of control and Zeb1^−/− mice at 2, 4, and 8 weeks post-induction.

(C) Summary graph of neuroblast changes over time.

(D) Representative images of NeuN⁺ granule neurons (arrows) at 4 weeks post-induction.

(E) Numbers of NeuN⁺tdTOM⁺ granule neurons at 4, 8, and 12 weeks post-induction.

(F) Summary graph showing granule neuron changes over time.

(G) 2D projection of a two-photon image z stack showing a typical granule cell filled with Alexa 488 via the patch-clamp recording electrode. Representative recordings from tdTOM expressing Zeb1^−/− (red) and control (blue) DGGCs.

(H) Scatterplots show resting membrane potential (R_m), input resistance (R_N), membrane time constant (τ_m), and membrane capacitance (C_m) for individual DGGCs overlaid with the mean for each group.

Additional graphs and Neurolucida traces are in Figure S3. Dots represent individual mice (minimum of two sections analyzed per animal; B and E), average values (C and F), or individual neurons (4 mice/genotype, H). Numerical data are shown as mean ± SEM. Scale bars: 20 μm (insets: 10 μm). See also Figures S2 and S3.