REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-ARHGEF2	Invitrogen	Cat#720323: RRID: AB_2633257
Rabbit anti-Cleaved caspase 3	Cell Signaling	Cat#9664S; RRID: AB_2070042
Chicken anti-Doublecortin (DCX)	Aves Lab	Cat#DCX; RRID: AB_2313540
Guinea pig anti-DCX	Millipore	Cat#AB2253; RRID: AB_1586992
Rabbit anti-ETV5	Invitrogen	Cat#PA5-30023; RRID: AB_2313540
Chicken anti-GFAP	Encor Biotechnology	Cat#CPCA-GFAP; RRID: AB_2109953
Mouse anti-GFAP	Sigma-Aldrich	Cat#G6171; RRID: AB_1840893
Rabbit anti-GFAP	Dako	Cat#Z0334; RRID: AB_10013382
Rabbit anti-INSC	Proteintech	Cat# 20973-1-AP; RRID: AB_10951111
Chicken anti-Ki67	Encor	Cat#CPCA-Ki67; RRID: AB_2637049
Rabbit anti-MCM2	Abcam	Cat#ab4461; RRID: AB_304470
Mouse anti-NeuN	Merck	Cat# MAB377; RRID: AB_2298772
Rabbit anti-RAB10	Cell Signaling	Cat#8127T; RRID: AB_10828219
Rabbit anti-S100β	NeoMarkers	Cat#AB-044-AO; RRID: AB_60518
Rabbit anti-TBR2	Abcam	Cat#ab23345; RRID: AB_778267
Rabbit anti-ZEB1	Sigma-Aldrich	Cat#HPA027524; RRID: AB_1844977
Goat anti-chicken Alexa Fluor 488	Invitrogen	Cat#A11039; RRID: AB_142924
Donkey anti-mouse Alexa Fluor 488	Invitrogen	Cat#A21202; RRID: AB_141607
Goat anti-mouse Alexa Fluor 405	Invitrogen	Cat#A31553; RRID: AB_221604
Donkey anti-rabbit Alexa Fluor 488	Invitrogen	Cat#A21206; RRID: AB_2535792
Donkey anti-rabbit Alexa Fluor 647	Invitrogen	Cat#A32795; RRID: AB_2762835
Donkey anti-rabbit Alexa Fluor 594	Invitrogen	Cat# A32754; RRID: AB_2762827
Donkey anti-rabbit IgG Fab fragments	Jackson ImmunoResearch	Cat# 711-007-003; RRID:AB_2340587
Bacterial and virus strains
Lentiviral plasmid for ETV5 expression (pLX_TRC311 ETV5)	Wang et al., 2017	Addgene plasmid # 74984; http://addgene.org/74984/ ; RRID:Addgene_74984
Chemical, peptides, and recombinant proteins
Optimal Cutting Temperature (OCT) Compound	Thermo Fisher Scientific	Cat#6502
Fish Skin Gelatin	Sigma Aldrich	Cat#G7765
Triton X-100	Thermo Fisher Scientific	Cat#13454259
10XPBS	Thermo Fisher Scientific	Cat#70011044
Hoechst-33342	Thermo Fisher Scientific	Cat#62249
Prolong Diamond Antifade Mountant	Thermo Fisher Scientific	Cat#P36961
DMEM/F12 with Glutamax	Thermo Fisher Scientific	Cat#11320033
recombinant human Insulin	Sigma-Aldrich	Cat#I5500
recombinant human Transferrin	Sigma-Aldrich	Cat#T8158
Putrescine	Sigma-Aldrich	Cat#P5780
Sodium Selenite	Sigma-Aldrich	Cat#S5261
Progesterone	Sigma-Aldrich	Cat#P8783
Bovine Serum Albumin (BSA) fraction V	Thermo Fisher Scientific	Cat#12737119
B27 supplement	Thermo Fisher Scientific	Cat#17504044
Penicillin-Streptomycin solution	Thermo Fisher Scientific	Cat#15140122
HBSS	Thermo Fisher Scientific	Cat#24020117
Papain	Roche	Cat#10108014001
Dispase II	Roche	Cat#165859
DNase I	Roche	Cat#10104159001
Hyaluronidase	Sigma-Aldrich	Cat#H3884
Trypsin	Sigma-Aldrich	Cat#T9201
Heparin	Sigma-Aldrich	Cat#H4784
recombinant human EGF	Peprotech	Cat#AF-100-15
recombinant human FGF-2	Peprotech	Cat#AF-100-18C
Poly-D-lysine	Sigma-Aldrich	Cat#P6407
Laminin	Sigma-Aldrich	Cat#L2020
Accumax	Thermo Fisher Scientific	Cat#00-4666-56
Tamoxifen	Sigma-Aldrich	Cat#T5648
4-Hydroxy-Tamoxifen	Sigma-Aldrich	Cat#SML1666
Corn oil	Sigma-Aldrich	Cat#C8267
Formaldehyde	Santa Cruz Biotechnology	Cat#sc-203049
Critical commercial assays
EdU Click 488 kit	Sigma-Aldrich	Cat#BCK-EDU488
SimpleChIP kit	Cell Signaling Technologies	Cat#91820
MyTaq Extract-PCR kit	Bioline	Cat#BIO-21127
MyTaq HS Red Mix 2X	Bioline	Cat#BIO-25047
Experimental models: Organisms/strains
Mouse: GLAST::CreERT2	M. Götz, Munich	N/A
Mouse: Rosa26lox-stop-lox-tdTomato (Ai9)	O. Sansom, Glasgow	N/A
Mouse: Zeb1flox/flox	T. Brabletz, Erlangen	N/A
Oligonucleotides
See Table S3 for oligonucleotide information.
Deposited data
Single-cell RNA-Seq data from RGL cells and astrocytes	Chai et al., 2017	GEO: GSE94010
Single-cell RNA-Seq data from dentate gyrus	Hochgerner et al., 2018	GEO: GSE95315
Chip-Seq data of glioblastoma cells	Rosmaninho et al., 2018	EMBL accession: E-MTAB-5541
Single-cell RNA-Seq data from cortex and hippocampus	Zeisel et al., 2015	GEO: GSE60361
Software and algorithms
ZEN microscopy software	Zeiss	https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html
ImageJ v2.52K	ImageJ NIH	https://imagej.net/
Neurolucida 360/Explorer	MBF Bioscience	N/A
GraphPad Prism v9.1	GraphPad Software, Inc.	https://www.graphpad.com/
The Tracing Tool (tTt) software v3.4.4	Hilsenbeck et al., 2016	https://bsse.ethz.ch/csd/software/ttt-and-qtfy.html