Antibodies |
|
Rabbit anti-ARHGEF2 |
Invitrogen |
Cat#720323: RRID: AB_2633257
|
Rabbit anti-Cleaved caspase 3 |
Cell Signaling |
Cat#9664S; RRID: AB_2070042
|
Chicken anti-Doublecortin (DCX) |
Aves Lab |
Cat#DCX; RRID: AB_2313540
|
Guinea pig anti-DCX |
Millipore |
Cat#AB2253; RRID: AB_1586992
|
Rabbit anti-ETV5 |
Invitrogen |
Cat#PA5-30023; RRID: AB_2313540
|
Chicken anti-GFAP |
Encor Biotechnology |
Cat#CPCA-GFAP; RRID: AB_2109953
|
Mouse anti-GFAP |
Sigma-Aldrich |
Cat#G6171; RRID: AB_1840893
|
Rabbit anti-GFAP |
Dako |
Cat#Z0334; RRID: AB_10013382
|
Rabbit anti-INSC |
Proteintech |
Cat# 20973-1-AP; RRID: AB_10951111
|
Chicken anti-Ki67 |
Encor |
Cat#CPCA-Ki67; RRID: AB_2637049
|
Rabbit anti-MCM2 |
Abcam |
Cat#ab4461; RRID: AB_304470
|
Mouse anti-NeuN |
Merck |
Cat# MAB377; RRID: AB_2298772
|
Rabbit anti-RAB10 |
Cell Signaling |
Cat#8127T; RRID: AB_10828219
|
Rabbit anti-S100β |
NeoMarkers |
Cat#AB-044-AO; RRID: AB_60518
|
Rabbit anti-TBR2 |
Abcam |
Cat#ab23345; RRID: AB_778267
|
Rabbit anti-ZEB1 |
Sigma-Aldrich |
Cat#HPA027524; RRID: AB_1844977
|
Goat anti-chicken Alexa Fluor 488 |
Invitrogen |
Cat#A11039; RRID: AB_142924
|
Donkey anti-mouse Alexa Fluor 488 |
Invitrogen |
Cat#A21202; RRID: AB_141607
|
Goat anti-mouse Alexa Fluor 405 |
Invitrogen |
Cat#A31553; RRID: AB_221604
|
Donkey anti-rabbit Alexa Fluor 488 |
Invitrogen |
Cat#A21206; RRID: AB_2535792
|
Donkey anti-rabbit Alexa Fluor 647 |
Invitrogen |
Cat#A32795; RRID: AB_2762835
|
Donkey anti-rabbit Alexa Fluor 594 |
Invitrogen |
Cat# A32754; RRID: AB_2762827
|
Donkey anti-rabbit IgG Fab fragments |
Jackson ImmunoResearch |
Cat# 711-007-003; RRID:AB_2340587
|
|
Bacterial and virus strains |
|
Lentiviral plasmid for ETV5 expression (pLX_TRC311 ETV5) |
Wang et al., 2017 |
Addgene plasmid # 74984; http://addgene.org/74984/ ; RRID:Addgene_74984 |
|
Chemical, peptides, and recombinant proteins |
|
Optimal Cutting Temperature (OCT) Compound |
Thermo Fisher Scientific |
Cat#6502 |
Fish Skin Gelatin |
Sigma Aldrich |
Cat#G7765 |
Triton X-100 |
Thermo Fisher Scientific |
Cat#13454259 |
10XPBS |
Thermo Fisher Scientific |
Cat#70011044 |
Hoechst-33342 |
Thermo Fisher Scientific |
Cat#62249 |
Prolong Diamond Antifade Mountant |
Thermo Fisher Scientific |
Cat#P36961
|
DMEM/F12 with Glutamax |
Thermo Fisher Scientific |
Cat#11320033 |
recombinant human Insulin |
Sigma-Aldrich |
Cat#I5500 |
recombinant human Transferrin |
Sigma-Aldrich |
Cat#T8158 |
Putrescine |
Sigma-Aldrich |
Cat#P5780 |
Sodium Selenite |
Sigma-Aldrich |
Cat#S5261 |
Progesterone |
Sigma-Aldrich |
Cat#P8783 |
Bovine Serum Albumin (BSA) fraction V |
Thermo Fisher Scientific |
Cat#12737119 |
B27 supplement |
Thermo Fisher Scientific |
Cat#17504044 |
Penicillin-Streptomycin solution |
Thermo Fisher Scientific |
Cat#15140122 |
HBSS |
Thermo Fisher Scientific |
Cat#24020117 |
Papain |
Roche |
Cat#10108014001 |
Dispase II |
Roche |
Cat#165859 |
DNase I |
Roche |
Cat#10104159001 |
Hyaluronidase |
Sigma-Aldrich |
Cat#H3884 |
Trypsin |
Sigma-Aldrich |
Cat#T9201 |
Heparin |
Sigma-Aldrich |
Cat#H4784 |
recombinant human EGF |
Peprotech |
Cat#AF-100-15 |
recombinant human FGF-2 |
Peprotech |
Cat#AF-100-18C |
Poly-D-lysine |
Sigma-Aldrich |
Cat#P6407 |
Laminin |
Sigma-Aldrich |
Cat#L2020 |
Accumax |
Thermo Fisher Scientific |
Cat#00-4666-56 |
Tamoxifen |
Sigma-Aldrich |
Cat#T5648 |
4-Hydroxy-Tamoxifen |
Sigma-Aldrich |
Cat#SML1666 |
Corn oil |
Sigma-Aldrich |
Cat#C8267 |
Formaldehyde |
Santa Cruz Biotechnology |
Cat#sc-203049 |
|
Critical commercial assays |
|
EdU Click 488 kit |
Sigma-Aldrich |
Cat#BCK-EDU488 |
SimpleChIP kit |
Cell Signaling Technologies |
Cat#91820 |
MyTaq Extract-PCR kit |
Bioline |
Cat#BIO-21127 |
MyTaq HS Red Mix 2X |
Bioline |
Cat#BIO-25047 |
|
Experimental models: Organisms/strains |
|
Mouse: GLAST::CreERT2
|
M. Götz, Munich |
N/A |
Mouse: Rosa26lox-stop-lox-tdTomato (Ai9) |
O. Sansom, Glasgow |
N/A |
Mouse: Zeb1flox/flox
|
T. Brabletz, Erlangen |
N/A |
|
Oligonucleotides |
|
See Table S3 for oligonucleotide information. |
|
Deposited data |
|
Single-cell RNA-Seq data from RGL cells and astrocytes |
Chai et al., 2017 |
GEO: GSE94010
|
Single-cell RNA-Seq data from dentate gyrus |
Hochgerner et al., 2018 |
GEO: GSE95315
|
Chip-Seq data of glioblastoma cells |
Rosmaninho et al., 2018 |
EMBL accession: E-MTAB-5541 |
Single-cell RNA-Seq data from cortex and hippocampus |
Zeisel et al., 2015 |
GEO: GSE60361
|
|
Software and algorithms |
|
ZEN microscopy software |
Zeiss |
https://www.zeiss.com/microscopy/int/products/microscope-software/zen.html |
ImageJ v2.52K |
ImageJ NIH |
https://imagej.net/ |
Neurolucida 360/Explorer |
MBF Bioscience |
N/A |
GraphPad Prism v9.1 |
GraphPad Software, Inc. |
https://www.graphpad.com/ |
The Tracing Tool (tTt) software v3.4.4 |
Hilsenbeck et al., 2016 |
https://bsse.ethz.ch/csd/software/ttt-and-qtfy.html |