Figure 3.

The Drp1 KI mouse model presents impaired S579 phosphorylation and increased mitochondrial elongation

(A) Scheme of the Drp1-targeting vector, the locus after insertion, and the Drp1 KI model after CRE recombination. hGHpA, human growth hormone polyadenylation signal.

(B) Tissue samples from WT and Drp1 KI mice were collected and used to evaluate Drp1 protein levels.

(C) WT and Drp1 KI male mice were treated with CL316,243 (1 mg/kg; 1 h) before collecting BAT and evaluating the protein lysates for Drp1 total and Drp1 phosphorylation levels (n = 3 mice per condition).

(D) Electron microscopy (EM) images from BAT (scale bar: 2 μm) of male WT and Drp1 KI mice.

(E) Mitochondrial profile area, mitochondrial volume density, and frequency distributions were calculated from EM images of the experiment in (D), corresponding to 20 independent images per BAT sample (n = 4 mice per genotype).

All values are presented as mean ± SEM. ^∗∗∗p < 0.001 (two-tailed Student’s t test) between the indicated groups. See also Figure S3.