FIG. 3.

Qualitative colony color assay showing the lrs and irs phenotypes of selected mutants. Freshly grown cells were scraped from YPD medium and streaked onto MLA medium for single colonies. Cells were grown 5 days before photographs were taken. lrs mutants produce white colonies and often display a hypersectoring phenotype; irs mutants produce a darker colony color, indicative of reduced MET15 expression compared to WT. lrs strains shown are WT (JS306), top1 (M122), rif1 (M158), cac1 (M179), sir2Δ (JS576), and lrs4Δ (JS574); irs strains shown are WT (JS311), hir3 (M487), rpd3 (M480), sin3Δ (JS493), sap30, M475, irs4 (M469), and tup1 (M419).