TABLE 2.
Mutants isolated from the genetic screen
| Mutation | Gene/ORF name | Isolate no. | Transposon insertion sitea | Transposon orientationb | Gene function | Additional phenotypes |
|---|---|---|---|---|---|---|
| lrs1 | RFC1/CDC44 | M65 | Promoter | Plus | DNA replication/processivity factor | Slow growth, long telomeres |
| lrs4 | YDR439W | M114 | 78/347 | Plus | Unknown | Slow growth |
| M169 | 78/347 | Plus | Slow growth | |||
| M345 | 78/347 | Plus | Slow growth | |||
| lrs5 | TOP1 | M122 | 10/769 | Plus | Topoisomerase I | |
| M154 | 267/769 | Minus | ||||
| M160 | 55/769 | Plus | ||||
| lrs6 | DPB3 | M155 | 72/201 | Plus | DNA polymerase ɛ/subunits C and C′ | Increased spontaneous mutation rate |
| M172 | 34/201 | Minus | ||||
| M182 | 72/201 | Plus | ||||
| M286 | 72/201 | Plus | ||||
| lrs7 | RIF1 | M98 | 444/1916 | Minus | Telomere length control | Long telomeres |
| M158 | 183/1916 | Plus | ||||
| lrs8 | CAC1 | M179 | 272/606 | Plus | Nucleosome assembly during replication | UV sensitive |
| lrs9 | CDC17/POL1 | M326 | 237/1468 | Minus | DNA polymerase α | Long telomeres |
| irs1 | SIR4 | M87 | 20/1358 | Minus | Silencing factor | Mating defective |
| irs2 | RPD3 | M480 | 135/433 | Minus | Histone deacetylase | Pleiotropicc |
| irs4 | YKR019C | M469 | 69/615 | Plus | Unknown | Slow growth on Pb2+ |
| irs8 | YMR263W/SAP30 | M475 | 107/201 | Plus | Sin3-associated protein | Pleiotropicc |
| irs10 | HIR3 | M390 | 150/1648 | Plus | Regulates histone expression | |
| M411 | 702/1648 | Plus | ||||
| M487 | 702/1648 | Plus | ||||
| M489 | 1496/1648 | Minus | ||||
| irs18 | TUP1 | M419 | Promoter | Minus | Transcriptional repressor | Flocculent |
| M420 | Promoter | Minus | Flocculent | |||
| M432 | 478/713 | Minus | Flocculent |
a
Position of transposon insertion relative to protein sequence in amino acids (insertion site/total amino acids). Exceptions are M65, M419, and M420, which had insertions within the promoter sequences.
b
Plus orientation means the lacZ gene of the transposon is in the same transcriptional orientation as the target ORF.
c
Cycloheximide sensitive, derepression of PHO5, ethidium bromide sensitivity (78), temperature sensitive at 37°C.