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Deletion screen by PCR amplification of the gpt coding sequence in G12-derived (A) and G10-derived (B) 6-TG-resistant cell lines. PCR products were separated on 1.2% agarose gels and stained with EtBr. The expected 561-bp PCR product is clearly visible in the control G10 and G12 cell lines and most of the examined variants. No products are shown in the negative control reaction (no template DNA) (lane N). M, molecular weight markers.
