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Genomic DNA methylation levels in 5-AzaC-induced 6-TGr variants of the G12 cell line
| Cell line | Genomic DNA methyl-ation level (% of control)a |
|---|---|
| G12 (control) | 100 |
| G12 6-TGr | |
| A13 | 59.4 ± 4.7 |
| A14 | 68.9 ± 1.9 |
| A15 | 80.10 ± 2.5 |
| A16 | 47.11 ± 4 |
| A18 | 50.4 ± 2.6 |
Methylation levels of genomic DNA were measured from duplicate samples by a methyl-accepting assay with CpG methylase (SssI) and calculated as follows: {1 + [(control − treated)/control]} × 100.
