TABLE 3.
CpG-methylated, nonmethylated, and partially methylated sites in the SV40 early promoter region of 5-AzaC-induced 6-TGr variants derived from the G10 and G12 cell linesa
| Site no.b | Methylationc in:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G10 variants
|
G12 variants
|
G12 | N37d | |||||||
| A2 | A3 | A5 | A6 | A7 | A14 | A15 | A23 | |||
| 1 | − | + | − | ND | − | ND | − | − | − | − |
| 2 | − | ± | − | ND | − | ND | − | − | − | − |
| 3 | ± | + | − | − | ± | − | − | ± | ± | ± |
| 4 | + | + | − | − | ± | − | − | ± | − | ± |
| 5 | + | + | − | − | + | − | ± | + | − | + |
| 6 | ± | + | − | − | ± | − | + | ± | − | ± |
| 7 | + | + | − | − | ± | ± | ± | ± | − | + |
| 8 | ± | ± | − | − | − | − | − | ± | − | ± |
| 9 | ± | ± | − | − | − | − | − | ± | − | ± |
| 10 | + | + | − | − | ± | − | ± | + | − | + |
| 11 | ± | ± | − | − | − | − | − | − | − | + |
| 12 | ± | ± | − | − | − | − | − | ± | − | + |
| 13 | ± | + | − | − | ± | − | ± | + | − | + |
| 14 | + | + | − | − | ± | − | + | + | − | + |
| 15 | + | + | − | − | + | ± | + | + | − | + |
| 16 | + | ± | − | − | ± | − | ± | + | ND | + |
| 17 | + | + | − | − | ± | − | ± | + | − | + |
| 18 | + | + | − | ± | ± | − | ± | ± | − | + |
Data were obtained by the sodium bisulfite genomic sequencing technique (5, 32) of independent cell lines. Direct PCR sequencing was done with an automated DNA sequencer to obtain a methylation map from the population average. Partial methylation at any site was observed by the presence of both a cytosine and a thymidine residue at the same sequencing position. Cytosines that are not associated with CpG dinucleotides have been converted to thymidines in all the samples.
The sites in this table correspond to the CpG sites marked in Fig. 5.
+, methylation; −, no methylation; ±, partial methylation.
N37 is a nickel-induced 6-TGr G12 variant, which was shown to be highly methylated in the gpt locus (22).