FIG. 2.

IRF-2 is the major protein binding to the Qp ISRE in type III cells. (A) Patterns of binding to the Qp ISRE in type I and type III cells. The probe was Qp ISRE labeled with [α-³²P]dCTP. Free probe (lane 1) and lysates from Akata (lane 2), CB95 (lanes 3 to 8), Sav I (lane 9), and Sav III (lane 10) cells were used for EMSA. Cold competitor (the Qp ISRE) was added in lane 4 at a 100-fold molar excess over the hot probe. The following antibodies were added: preimmunization serum (lane 5), IRF-7 antiserum (lane 6), α-IRF-1 antiserum (lane 7), and α-IRF-2 antiserum (lane 8). Arrows with question mark indicate a band found primarily in type I cell lysates. (B) Sequence comparison between a sequence selected randomly by IRF-2 binding and the Qp ISRE.