FIGURE 4.

In vivo mouse experiments with hyaluronidase. (a) Pathology (PAS, HABP stain, and EM) of mouse kidney 2 h after injection with or without hyaluronidase. Control mice are injected with saline alone. Scale bars in PAS and HABP stain = 20 μm, Scale bars in EM = 200 nm. (b‐g) Fenestral density, fenestral diameter, foot process width, hyaluronan concentrations in serum, serum creatinine concentration, and albuminuria with and without hyaluronidase administration. Intraperitoneal injection with hyaluronidase (Hya, Hyaluronidase group) increased albuminuria (f) associated with loss of glomerular hyaluronan expression (a) and increase in serum hyaluronan levels (e). No differences are evident in fenestral density (b), fenestral diameter (c), and foot process width (d). n.s., not significant