Fig. 3.

In vitro PVES/mRNA complexes transfection and expression in HeLa, Vero and DC2.4 cells. After 24 h transfection, expression of eGFP mRNA were analyzed by fluorescence microscope and flow cytometry. (A) PVES/mRNA transfection efficiencies in HeLa and Vero cells at N/P ratios of 32 and 40; PVES/mRNA transfection efficiency in DC2.4 cells at N/P ratios of 32. Scale bar 200 μm in HeLa cells and 500 μm in Vero and DC2.4 cells. (B) eGFP expression efficiency was evaluated by the number of GFP positive cells measured by flow cytometry. PEI 25 k and PEI 1.8 k were used as controls. Data were shown as mean ± SEM. Significance was calculated using unpaired t-test (****, P < 0.0001).