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(A) Add the zebrafish embryo to the small well created by the glass bottom in the 35 mm dish. (B) Add agarose layer 1 to the small well to cover the embryo. (C) Carefully place a cover glass over the small well. (D) Add agarose layer 2 on the whole bottom of the 35 mm dish. (E) Add E3 to the dish. (F) Schematic drawing of a cross section of the mounting set up. (G) Microscope image (5x objective) of the zebrafish embryo in the final montage.
