Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
S1 nuclease protection assay of CUP1 transcription induction by copper. Total RNAs were isolated from wild-type (WT) (KT1099), glc7 (KT1098), and gac1 (KT1100) strains. Cultures were grown to mid-exponential phase at 30°C and either harvested (−) or induced by 500 μM CuSO4 for 45 min (+). The RNAs were annealed to an oligonucleotide probe complementary to the CUP1 gene.
