TABLE I.

Parameter settings

Symbol	Parameter	Values
Δt	Image period (discrete time step)	100 ms
T	Number of images per dataset	400
tc	time of change	200s
P	Number of pixels per squared image	25
$D_x^1$	Diffusion coeff. in x direction before tc	0.001 μm2/s
$D_x^2$	Diffusion coeff. in x direction after tc	0.002 μm2/s
$D_y^1$	Diffusion coeff. in y direction before tc	0.001 μm2/s
$D_y^2$	Diffusion coeff. in y direction after tc	0.002 μm2/s
Δx	Length of unit pixel	100 nm
Δy	Width of unit pixel	100 nm
λ	Emission wavelength	540 nm
NA	Numerical aperture	1.2