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. Author manuscript; available in PMC: 2021 Sep 2.
Published in final edited form as: ACS Chem Neurosci. 2018 Apr 16;9(6):1283–1289. doi: 10.1021/acschemneuro.8b00096

Figure 2.

Figure 2.

Binding of [3H]MCL-536 to dopamine D2 receptor expressed in CHO membranes (n = 5). Membranes of CHO cells were incubated with [3H]MCL-536 in increasing concentrations with or without 100 nM NPA. The total binding of [3H]MCL-536 minus [3H]MCL-536 in the presence of 100 nM NPA yielded a nonsaturable, nonspecific component (“apomorphine site”) and a saturable, specific component (total high- and low-affinity state D2 receptors). The dissociation constant, Kd, of [3H]MCL-536 for the D2 receptors occurred at the concentration where 50% of the receptors were occupied.