FIGURE 1.

Purkinje cell loss and motor deficits in Tmem106b−/− mice. (A) Representative images of Hematoxylin and eosin (H&E) stain and Calbindin and IP3R immunostaining in cerebellums of 15‐month‐old Tmem106b+/+ (WT) and Tmem106b−/− (KO) mouse brains. (B–E) Quantification of Purkinje cells in the cerebellums of WT, Tmem106b+/− (Het), and KO mouse brains at indicated ages (n = 4–17 per group). Graphs represent the mean ± SD. Data were analyzed by One‐way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. NS, not significant, ****p < 0.0001. (F) Representative images of Gfap and Iba‐1 immunostaining in cerebellums of 15‐month‐old Tmem106b WT and KO mouse brains. (G–H) Quantification of Gfap (G) and Iba‐1(H) immunointensities in the cerebellums of WT and KO mouse brains in F (n = 3–4 per group). Graphs represent the mean ± SD. Data were analyzed by Student’s t‐test. *p < 0.05, **p < 0.01. (I) Dowel test for Tmem106b WT, Het, and KO mice (n = 14–16 per group). Graphs represent the mean ± SD. Data were analyzed by One‐way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. NS, not significant, **p < 0.01