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. 2021 Aug 23;17(8):e1009775. doi: 10.1371/journal.pgen.1009775

Fig 3. Deletion of the 26-167bp or 515-648bp regions of the mex-3 3´UTR does not alter MEX-3 expression pattern or affect fertility.

Fig 3

(A) DIC and fluorescence images of the homozygous mex-3(spr6) mutant animals. (B) DIC and fluorescence images of the homozygous mex-3(spr7) mutant animals. (C) quantitative analysis of the fluorescence intensity in the homozygous mex-3(spr6) mutant animals compared to that of wild type GFP::MEX-3 (n = 16). (D) quantitative analysis of fluorescence intensity in the homozygous mex-3(spr7) mutant animals compared to that of wild type GFP::MEX-3 (n = 21). (E) brood size assay of homozygous mex-3(spr6) mutant animals at 20°C.There is no reduction in the brood size. (F) brood size assay of homozygous mex-3(spr7) mutant animals at 20°C. Each dot in panels E and F represents the brood size of an individual animal. In each panel, data from three biological replicates are shown in the graph. All p-values for panels C and D are reported in S4 Table. P-values in panels E and F are from a Kolmogorov-Smirnov test. All images taken at 40x magnification. Scale bar = 30 μm.