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. 2021 Aug 23;17(8):e1009775. doi: 10.1371/journal.pgen.1009775

Fig 6. Polyadenylation contributes to regulation of MEX-3 in the germline.

Fig 6

(A) DIC and fluorescence images of wild type GFP::MEX-3 animals from the control RNAi. (B) DIC and fluorescence images of the gfp::mex-3 3´UTR transgenic reporter strain from the control RNAi. (C) a model representing the known mechanism of GLD-2 and GLD-3 mediated polyadenylation. GLD-2, which lacks an RNA-binding domain, is thought to utilize the RBPs GLD-3 or RNP-8 in order to bind its target mRNAs. GLD-3 also exhibits functions independent of its role with GLD-2. (D) DIC and fluorescence images after gld-2 knockdown. GFP::MEX-3 was significantly reduced in the distal mitotic end. (E) DIC and fluorescence images after gld-2 knockdown in the transgenic reporter strain. Transgene expression was significantly reduced in the distal mitotic end. (F) quantitative analysis of fluorescence intensity after gld-2 knockdown in the GFP::MEX-3 strain (n = 15/15). (G) quantitative analysis of fluorescence intensity after gld-2 knockdown in the gfp:mex-3 3´UTR strain (n = 14/14). (H) DIC and fluorescence images after gld-3 knockdown in the GFP::MEX-3 strain. GFP::MEX-3 expression was increased in the pachytene and oogenic regions. (I) DIC and fluorescence images after gld-3 knockdown in the gfp::mex-3 3´UTR transgenic reporter strain. Transgene expression was not increased in the meiotic region but was increased in the oogenic region. (J) quantitative analysis of fluorescence intensity after gld-3 knockdown in the GFP::MEX-3 strain (n = 7/12). (K) quantitative analysis of fluorescence intensity after gld-3 knockdown in the gfp:mex-3 3´UTR strain (n = 10/10). (*) indicates statistical significance, adjusted p-value ≤ 0.05. All p-values for this figure are reported in S7 and S8 Tables. Scale bar = 30 μm.