Representative images of the germarium (A), Stage 3 oocytes (B), Stage 3 nurse cells (C) and different stages of follicle cells (D) from D. melanogaster ovarioles. Shown (from left to right) are the DAPI staining (blue) and immunofluorescent stainings using anti-HP1a (white), anti-CENP-C (green), anti-HMR (red) and the merge of either HP1a and HMR or CENP-C and HMR channels. For better illustration the nuclear boundary is labelled with a dashed white line, the HP1a positive heterochromatic area with a red line and the centromere with a white arrow. Different cell types of the germarium were identified based on their size and the position within the germarium and labelled accordingly. Size bar indicates 10 μm in (A), 5 μm in (B) and (C) and 15 μm in (D).