Fig 7. Deletion of Hmr’s C-terminus results in failure to rescue hybrid lethality.

(A) Schematic representation of crosses performed for hybrid viability suppression assays. Crosses between D. melanogaster mothers carrying Hmr transgenes in a Df(1)Hmr—background (Hmr^ko; Hmr*) and wild type D. simulans males (C167.4 and w⁵⁰¹). Suppression of hybrid male viability is analyzed within the transgene-carrying offspring only. Hmr* refers to any transgene used in this work. (B) HMR C-terminus is necessary for HMR lethal function in hybrid males. Suppression of hybrid males viability was measured as percentage of viable adult males in the total hybrid adult offspring. Crosses from non-complemented Hmr^ko mothers were used as control. Dots represent individual biological replicates. Wilcoxon rank sum test was used for pairwise comparisons with Hmr⁺ as a reference group and fdr for multiple testing adjustment (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001). For details refer to S5 Table.