FIG. 3.

CCCP and DNP enhance the Fas apoptosis signal in CEM cells. CEM cells (2 × 10⁵ per well) were incubated for 3 h in 96-well plates in 200 μl of complete medium or with various concentrations of CCCP (top panels) or DNP (bottom panels) as indicated either without (○) or with (●) Anti-Fas MAb (100 ng/ml). Aliquots of the cultures were then assayed for apoptotic changes either morphologically (left panels) or after staining with propidium iodide for DNA content by flow cytometry (right panels). Note that the concentrations of CCCP required are lower than for Jurkat cells. Experiments for these titration curves were performed three times with very similar results.