TABLE 2.
Effect of overexpression of HAL4 and HAL5 on the intracellular cation concentrations of cells incubated with LiCl and NaCla
| Strain | Plasmid | No salt, [K+]ib | 0.1 M LiClb
|
1 M NaClb
|
||
|---|---|---|---|---|---|---|
| [Li+]i | [K+]i | [Na+]i | [K+]i | |||
| wt | YEp351 | 330 (±30) | 69 (±3) | 383 (±6) | 283 (±10) | 92 (±5) |
| YEpHAL4 | 330 (±20) | 46 (±4) | 399 (±3) | 225 (±9) | 151 (±8) | |
| YEpHAL5 | 351 (±12) | 53 (±4) | 410 (±5) | 250 (±50) | 165 (±7) | |
| ena1-4 | YEp351 | 125 (±12) | 331 (±10) | 199 (±13) | ||
| YEpHAL4 | 113 (±6) | 300 (±30) | 260 (±20) | |||
| YEpHAL5 | 120 (±20) | 310 (±40) | 289 (±11) | |||
a
Strain W303-1A (wt), and its ena1-4 derivative (SKY697) were transformed with either empty plasmid YEp351 or YEp351 carrying HAL4 (YEpHAL4) or HAL5 (YEpHAL5). Cells were grown in YPD to exponential phase and treated for 3 h with the indicated concentrations of either LiCl or NaCl.
b
The intracellular concentrations (millimolar units) of potassium ([K+]i), lithium ([Li+]i), and sodium ([Na+]i) were determined as described in Materials and Methods. Results are the averages of three determinations (± standard deviations).