Fig. 2.

Assessment of linearity of ECL assay (anti-PT, anti-FHA, anti-PRN, and anti-FIM IgG EU/mL). (A) anti-PT ECL using WWO-2-043; (B) anti-PT ECL using NIBSC 06/140; (C) anti-FHA ECL using WWO-2-043; (D) anti-FHA ECL using NIBSC 06/140; (E) anti-PRN ECL using WWO-2-043; (F) anti-PRN ECL using NIBSC 06/140; (G) anti-FIM ECL using WWO-2-043; (H) anti-FIM ECL using NIBSC 06/140. The dashed red line shows the line of perfect linear agreement. The blue or green circles and the solid blue or green line show the actual data points and the associated trendline for observed versus expected results for the indicated DTP-ECL antigen using the indicated reference standard as the source of the samples