Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Mir146b | Ensembl | ENSMUSG00000070127 | |
| Strain, strain background (M. musculus) | Mir146b flox/flox | This paper (see'Materials and methods' and Figure 2—figure supplement 1) | ENSMUSG00000070127 | Mouse line |
| Strain, strain background (M. musculus, female) | C57Bl/6 | NIA Aged Rodent Colony | C57BL/6 RRID:SCR_007317 |
|
| Strain, strain background (M. musculus, female) | C57Bl/6 | The Jackson Laboratory |
000664 RRID:IMSR_JAX:000664 |
|
| Strain, strain background (M. musculus, female) | Lyz2 Cre | The Jackson Laboratory |
004781 RRID:IMSR_JAX:004781 |
Mouse line |
| Cell line (M. musculus) | Primary thioglycollate-elicited macrophages | This paper (see 'Materials and methods') | C57Bl/6, 3–30 months; Control or cKO, 3–17 months |
Freshly isolated |
| Cell line (M. musculus) | Primary Bone Marrow-Derived Macrophages | This paper (see 'Materials and methods') | C57Bl/6, 3–30 months; Control or cKO, 3 months | Freshly isolated |
| Transfected construct (M. musculus) | mirVana Mir146b inhibitor |
Life Technologies | 4464084 | |
| Transfected construct (M. musculus) | mirVana negative control | Life Technologies | 4464076 | |
| Transfected construct (M. musculus) | miRCURY LNA Mir146b mimic | Qiagen | Cat # 3391173 GeneGlobe ID: YM00472354-ABD |
5’-UGAGAACUGAAUUCCAUAGGCU-3’ |
| Transfected construct (M. musculus) | miRCURY LNA negative control A | Qiagen | Cat# 300611-04 | 5’-/F6-FAM/AACACGTCTATACGC-3’ |
| Sequence-based reagent | hsa-Mir146b-5p | Qiagen | Cat# 339306 GeneGlobe ID: YP00204553 |
miRNA expression |
| Sequence-based reagent | hsa-Mir146a-5p | Qiagen | Cat# 339306 GeneGlobe ID: YP00204688 |
miRNA expression |
| Sequence-based reagent | U6 snRNA | Qiagen | Cat# 339306 GeneGlobe ID: YP00203907 | miRNA expression |
| Sequence-based reagent | hsa-Mir-15a-5p | Qiagen | Cat# 339306 GeneGlobe ID: YP00204066 |
miRNA expression |
| Sequence-based reagent | hsa-Mir-18a-5p | Qiagen | Cat# 339306 GeneGlobe ID: YP00204207 |
miRNA expression |
| Sequence-based reagent | hsa-Mir-22-5p | Qiagen | Cat# 339306 GeneGlobe ID: YP00204255 |
miRNA expression |
| Sequence-based reagent | hsa-Mir-29a-5p | Qiagen | Cat# 339306 GeneGlobe ID: YP00204430 |
miRNA expression |
| Sequence-based reagent | mmu-Mir-362-5p | Qiagen | Cat# 339306 GeneGlobe ID: YP00205073 |
miRNA expression |
| Sequence-based reagent | hsa-Mir-423-5p | Qiagen | Cat# 339306 GeneGlobe ID: YP00205624 |
miRNA expression |
| Sequence-based reagent | QuantiGene Mir146b-5p |
Affymetrix | Cat# SM-10013-01 | miRNA expression |
| Sequence-based reagent | QuantiGene U6 |
Affymetrix | Cat# SR-19005-01 | miRNA expression |
| Sequence-based reagent | Universal Neo F | This paper (see Supplementary file 2) | Genotyping forward primer | TGC TCC TCG CGA GAA AGT ATC CAT CAT GGC |
| Sequence-based reagent | Universal Neo R | This paper (see Supplementary file 2) | Genotyping reverse primer | CGC CAA GCT CTT CAG CAA TAT CAC GGG TAG |
| Sequence-based reagent | Mir146b Neo F | This paper (see Supplementary file 2) | Genotyping forward primer | ATA TCT GGC CCA CCA GGA ACA CAT |
| Sequence-based reagent | Mir146b Neo R | This paper (see Supplementary file 2) | Genotyping reverse primer | AGC CTC TGT GTG TGC TTG TGA CAT |
| Sequence-based reagent | LoxP F | This paper (see Supplementary file 2) | Genotyping forward primer | TAA CGG CAT TAG CCA CCA CCT TCA |
| Sequence-based reagent | LoxP R | This paper (see Supplementary file 2) | Genotyping reverse primer | TGG GTT ATG TAG GGA TCC TGG GTT |
| Sequence-based reagent | Flp/o FWD | This paper (see Supplementary file 2) | Genotyping forward primer | ATA GCA GCT TTG CTC CTT CG |
| Sequence-based reagent | Flp/o REV | This paper (see Supplementary file 2) | Genotyping reverse primer | TGG CTC ATC ACC TTC CTC TT |
| Sequence-based reagent | Flp/o Internal FWD | This paper (see Supplementary file 2) | Genotyping forward primer | CTA GGC CAC AGA ATT GAA AGA TCT |
| Sequence-based reagent | Flp/o Internal Rev | This paper (see Supplementary file 2) | Genotyping reverse primer | GTA GGT GGA AAT TCT AGC ATC ATC C |
| Sequence-based reagent | Lyz2Cre F | This paper (see Supplementary file 2) | Genotyping forward primer | GTA GGT GGA AAT TCT AGC ATC ATC C |
| Sequence-based reagent | Lyz2Cre R | This paper (see Supplementary file 2) | Genotyping reverse primer | TGG GTT ATG TAG GGA TCC TGG GTT |
| Sequence-based reagent | Actinb gene expression assay | Life Technologies | Cat# 4352933E Assay ID: Mm00607939_s1 |
mRNA expression |
| Sequence-based reagent | Gapdh gene expression assay | Life Technologies | Cat# 4331182 Assay ID: Mm99999915_g1 |
mRNA expression |
| Sequence-based reagent | Nos2 gene expression assay | Life Technologies | Cat# 4331182 Assay ID: Mm00440502_m1 |
mRNA expression |
| Sequence-based reagent | Mmp9 gene expression assay | Life Technologies | Cat# 4331182 Assay ID: Mm00442991_m1 |
mRNA expression |
| Sequence-based reagent | Il6 gene expression assay | Life Technologies | Cat# 4331182 Assay ID: Mm00446191_m1 |
mRNA expression |
| Sequence-based reagent | Il1b gene expression assay | Life Technologies | Cat# 4331182 Assay ID: Mm01336189_m1 |
mRNA expression |
| Sequence-based reagent | Ccl2 gene expression assay | Life Technologies | Cat# 4331182 Assay ID: Mm00478593_m1 |
mRNA expression |
| Sequence-based reagent | Cd163 gene expression assay | Life Technologies | Cat# 4331182 Assay ID: Mm00474091_m1 |
mRNA expression |
| Sequence-based reagent | Arg1 gene expression assay | Life Technologies | Cat# 4331182 Assay ID: Mm00475988_m1 |
mRNA expression |
| Sequence-based reagent | Il10 gene expression assay | Life Technologies | Cat# 4331182 Assay ID: Mm00439614_m1 |
mRNA expression |
| Chemical compound, drug | 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide | Millipore-Sigma | Cat# M5655 | MTT |
| Chemical compound, drug | Thioglycollate | Millipore-Sigma | Cat# T9032 | |
| Antibody | CD11b (Rat Monoclonal; (M1/70), eFluor 450) | ThermoFisher | 48-0112-82 RRID:AB_1582236 |
Flow (1:100) |
| Antibody | F4/80 (Rat Monoclonal; (BM8), APC) | ThermoFisher | 17-4801-82 RRID:AB_2784648 |
Flow (1:100) |
| Commercial assay or kit | RNeasy Mini Kit | Qiagen | Cat# 74104 | RNA extraction |
| Commercial assay or kit | mirVANA RNA Isolation kit | Life Technologies | Cat# AM1560 | RNA extraction |
| Commercial assay or kit | QuantiGene 2.0 miRNA Assay | Affymetrix | Cat# QS0008 | |
| Commercial assay or kit | miRCURY LNA RT kit | Qiagen | Cat# 339340 | |
| Commercial assay or kit | High Capacity cDNA Reverse Transcription Kit | Life Technologies | Cat# 4368813 | |
| Commercial assay or kit | ApopTag Fluorescein In Situ TUNEL labeling Kit | Millipore-Sigma | Cat# S7110 | |
| Commercial assay or kit | Seahorse XF Cell Mito Stress test kit | Agilent Technologies | Cat# 103708-100 | |
| Software, algorithm | ATM Image Capture Engine V602 software | Advanced Microscopy Techniques | ||
| Software, algorithm | Cofactor EXP software package | CoFactor Genomics | ||
| Software, algorithm | STAR aligner | GitHub | RRID:SCR_004463 | |
| Software, algorithm | Quant3p | GitHub | RRID:SCR_021236 | |
| Software, algorithm | Phantasus | Bioconductor | RRID:SCR_006442 | |
| Software, algorithm | Cellranger | 10x Genomics | RRID:SCR_017344 | |
| Software, algorithm | Monocle3 | GitHub | RRID:SCR_018685 | |
| Software, algorithm | Graphpad Prism | Graphpad | RRID:SCR_002789 | |
| Other | TopCount NTX counter | Perkin Elmer | ||
| Other | Spark multi-mode plate reader | Tecan | ||
| Other | Seahorse XF96 Extracellular Flux Analyzer | Agilent Technologies | RRID:SCR_013575 | |
| Other | StepOne Plus Real-Time PCR System | Life Technologies | RRID:SCR_015805 | |
| Other | Viia 7 Real-Time PCR System | Life Technologies | RRID:SCR_019582 | |
| Other | Illumina GAII sequencing platform | CoFactor Genomics | ||
| Other | Illumina HiSeq2500 sequencing platform | Centre for Applied Genomics; SickKids | RRID:SCR_001840 | |
| Other | NovaSeq S4 sequencing platform | GTAC; Washington University | RRID:SCR_001030 | |
| Other | BD FACSCanto Flow Cytometry System | BD Biosciences | RRID:SCR_018055 |