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. 2021 Aug 2;10:e66234. doi: 10.7554/eLife.66234

Figure 5. Meiotic recombinase Dmc1 is required for pseudosexual reproduction.

(A) Light microscopy images showing the impact of dmc1 mutation on sexual and pseudosexual reproduction in C. neoformans. Scale bar, 100 µm. (B) A graph showing quantification (n=3) of sporulation events in multiple crosses with dmc1Δ mutants. At least 3000 basidia were counted in each experiment.

Figure 5.

Figure 5—figure supplement 1. Dmc1 deletion leads to severe sporulation defects in both sexual and pseudosexual reproduction.

Figure 5—figure supplement 1.

Light microscopy images showing the phenotype of DMC1 deletion in (A) H99α×KN99a unilateral crosses as well as bilateral mutant crosses and (B) VYD135α×KN99a dmc1Δ unilateral and bilateral crosses. The deletion of DMC1 led to a reduction in sporulating basidia in bilateral mutant crosses.
Figure 5—figure supplement 2. Meiotic regulator Dmc1 is required for pseudosexual reproduction.

Figure 5—figure supplement 2.

A cross between a GFP-H4 tagged VYD135α strain and an mCherry-H4 tagged KN99a dmc1Δ mutant revealed that Dmc1 is required for pseudosexual reproduction events. The majority of the KN99a dmc1Δ nucleus-containing basidia failed to produce spore chains (top two rows and bottom rows). While all 11 observed basidia with VYD135α nuclei produced spores, only 2 out of 19 observed basidia with KN99a dmc1Δ nuclei produced spores. One of these two is represented in the third row. Scale bar, 10 µm.