Skip to main content
. 2021 Sep 1;41(35):7479–7491. doi: 10.1523/JNEUROSCI.0476-21.2021

Figure 2.

Figure 2.

MAO-B inhibition facilitates α-syn secretion via the non-classical ABC transporter-mediated pathway. Western blot analysis of TCA-precipitated CM and cell lysates with Syn-1 antibody. β-Actin was used as a loading control. A, B, Effect of brefeldin A on MAO-B inhibitor-induced α-syn secretion. wt-aS/SH cells were treated with 1 mm selegiline (A) or 1 mm rasagiline (B) in the absence and presence of 7 μm brefeldin A for 6 h (n = 3 per group). C, D, Effect of probenecid on MAO-B inhibitor-induced α-syn secretion. wt-aS/SH cells were treated with 1 mm selegiline (C; n = 4; F(2,9) = 65.197, p < 0.001, ANOVA) or 1 mm rasagiline (D; n = 3; F(2,6) = 10.362, p = 0.011, ANOVA) in the absence and presence of 10 μm probenecid for 24 h. E, F, Effect of glyburide on MAO-B inhibitor-induced α-syn secretion. After pretreating wt-aS/SH cells with vehicle or 5 μm glyburide for 24 h, cells were treated with 1 mm selegiline (E; n = 3; F(2,6) = 77.896, p < 0.001, ANOVA) or 1 mm rasagiline (F; n = 3; F(2,6) = 64.275, p < 0.001, ANOVA) in the absence and presence of 5 μm glyburide for 24 h. C–F, Middle graphs show the ratios of relative intensities of α-syn signals to controls. A–F, Right graphs show LDH release ratios to lysis buffer-treated cells (n = 6). Data represent mean ± SD. Data were analyzed by one-way ANOVA with Bonferroni's post hoc test; *p < 0.05, **p <0.01, ***p <0.001. Brefeldin A, Bre; Glyburide, Gly; lysis buffer, Ly. buffer; probenecid, Pro; rasagiline, Ras; secreted α-syn, Sec. αS; selegiline, Sel.