Table 2.
The analysis results of the orthogonal experiment.
| No. | V1 (ml) | C1 (μg/ml) | Extraction rate (%) | V2 (ml) | V2 : V3 | V3 (ml) | C2 (μg/ml) | LLE rate (%) | Transfer efficiency (%) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 7 | 16.59 | 87.74 | 4.0 | 1 : 3 | 12.0 | 5.36 | 99.03 | 86.89 |
| 2 | 2 : 3 | 6.0 | 10.63 | 94.94 | 83.30 | ||||
| 3 | 4 : 3 | 3.0 | 18.88 | 91.27 | 80.08 | ||||
|
| |||||||||
| 4 | 3.5 | 30.91 | 81.67 | 2.0 | 1 : 3 | 6.0 | 9.97 | 95.80 | 78.24 |
| 5 | 2 : 3 | 3.0 | 20.23 | 104.64 | 85.46 | ||||
| 6 | 4 : 3 | 1.5 | 41.00 | 103.17 | 84.26 | ||||
|
| |||||||||
| 7 | 2.0 | 29.84 | 43.30 | 1.0 | 1 : 3 | 3.0 | 17.84 | 179.33 | 77.65 |
| 8 | 2 : 3 | 1.5 | 23.85 | 119.90 | 51.92 | ||||
| 9 | 4 : 3 | 0.75 | 48.85 | 122.78 | 53.16 | ||||
C: the concentration of sibutramine hydrochloride in the detection solution. LLE rate: the extraction rate of liquid-liquid extraction step. Transfer efficiency: the efficiency of the sibutramine transfer from the sample to the test liquid.