Table 3.
Δlog10 colony changes of P. aeruginosa and E. coli strains obtained by the time-kill assay at different time points after treatment with sub-MIC to 2 x MICs of A22, in relation to the initial inoculum.
| Strains | Fold MIC | Colony changes (Δlog10 cfu/mL) vs. initial inoculum at | |||
|---|---|---|---|---|---|
| 3 h | 6 h | 9 h | 24 h | ||
| P. aeruginosa | 0.25 | +0.96 (⦰) | +0.39 (⦰) | +0.87 (⦰) | +5.41 (⦰) |
| 0.5 | +0.14 (⦰) | −0.54 (Bs) | −0.97 (Bs) | +3.23 (⦰) | |
| 1 | −2.36 (Bs) | −3.05 (Bc) | −3.12 (Bc) | −5 (Bc) | |
| 2 | −5.04 (Bc) | −5.04 (Bc) | −5.04 (Bc) | −5.04 (Bc) | |
|
| |||||
| E. coli | 0.25 | +0.63 (⦰) | +0.7 (⦰) | +0.97 (⦰) | +3.63 (⦰) |
| 0.5 | +0.63 (⦰) | +0.49 (⦰) | +0.15 (⦰) | +2.5 (⦰) | |
| 1 | +0.37 (⦰) | +0.35 (⦰) | +0.14 (⦰) | −2.34 (Bs) | |
| 2 | −0.78 (Bs) | −1.58 (Bs) | −2.36 (Bs) | −4.85 (Bc) | |
+: increase growth; −: decrease growth; bacteriostatic (Bs), bactericidal (Bc): <3log10 and ≥3log10 reduction in cfu/mL, respectively, relative to the initial inoculum; (⦰): no effect.