Skip to main content
. 2021 Aug 25;2021:9987219. doi: 10.1155/2021/9987219

Figure 2.

Figure 2

MiR-21-3p plays a major role in adipose browning. (a) Adipocytic differentiation of preadipocytes isolated from WT or miR-21 KO mice and oil red O staining of mature adipocytes after differentiation induction, scale bar = 50 μm. Preadipocytes from miR-21 KO mice were induced to differentiate into mature adipocytes and then transfected with mimics NC, miR-21a-3p mimics, or miR-21a-5p mimics. (b) The relative miR-21-3p and miR-21-5p expressions were detected by qRT-PCR in mature adipocytes. (c) UCP1 expression was analyzed by western blot and quantified by Scion Image software. (d) Representative images and quantification of UCP1 expression were detected by immunofluorescence in mature adipocytes, scale bar = 50 μm. UCP1 (red) is shown; nuclei are stained by DAPI (blue). The data are presented as the mean ± SD of three independent experiments. P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.